Abstract
Rho-type small GTPases (Rho GTPases) play central roles in various cellular events. Rho GTPases are often activated locally on the plasma membrane, forming plasma membrane domains, which induce downstream signaling. We describe an experimental procedure designed for inducing the production of de novo plasma membrane domains using Arabidopsis ROP11 GTPase. Introduction of ROP11 and its activator and inactivator into the tobacco leaf epidermis leads to formation of ROP11-activated plasma membrane domains on the plasma membrane. Effectors and marker genes can also be introduced alongside ROP11. This reconstruction system allows identifying molecules regulating Rho GTPase polarization.
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Acknowledgments
This work was supported by grants from MEXT KAKENHI (grant no. 16H01247 to YO and 15H05958 to HF), the JSPS KAKENHI (grant no. 16H06172 to YO and 16H06377 to HF), and the Naito Foundation to HF. YN is a special joint researcher of the National Institute of Genetics.
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Oda, Y., Nagashima, Y., Fukuda, H. (2018). Reconstruction of ROP GTPase Domains on the Plasma Membrane in Tobacco Leaves. In: Rivero, F. (eds) Rho GTPases. Methods in Molecular Biology, vol 1821. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8612-5_26
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DOI: https://doi.org/10.1007/978-1-4939-8612-5_26
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Publisher Name: Humana Press, New York, NY
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