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Micropipette Aspiration of Oocytes to Assess Cortical Tension

  • Janice P. Evans
  • Douglas N. Robinson
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1818)

Abstract

Just as it is important to understand the cell biology of signaling pathways, it is valuable also to understand mechanical forces in cells. The field of mechanobiology has a rich history, including study of cellular mechanics during mitosis and meiosis in echinoderm oocytes and zygotes dating back to the 1930s. This chapter addresses the use of micropipette aspiration (MPA) to assess cellular mechanics, specifically cortical tension, in mammalian oocytes.

Key words

Cellular mechanics Signaling pathways Mitosis Meiosis 

Notes

Acknowledgments

Our work on cortical tension in oocytes has been supported by Grant #HD074773 from the NIH, with related work in the Robinson lab supported by Grant #GM066817 and in the Evans lab by Grant # HD090624. We are especially grateful to Dr. Stephanie Larson and Dr. Hyo Lee, who led the way in the development of this methodology of use with oocytes, with assistance from Dr. Yee-Seir Kee.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, Bloomberg School of Public HealthJohns Hopkins UniversityBaltimoreUSA
  2. 2.Department of Cell Biology, School of MedicineJohns Hopkins UniversityBaltimoreUSA
  3. 3.Department of Pharmacology and Molecular Sciences, School of MedicineJohns Hopkins UniversityBaltimoreUSA

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