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A Pig Model of Myocardial Infarction: Catheter-Based Approaches

  • Olympia Bikou
  • Shin Watanabe
  • Roger J. Hajjar
  • Kiyotake Ishikawa
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1816)

Abstract

Despite enormous efforts in treating myocardial infarction (MI) and subsequent heart failure, the recent statistics from the American Heart Association evidently show that there still remains room for improvements. To develop and translate new therapeutics toward clinics, large animal models that allow us to test new therapies in human-like conditions are of extraordinary importance. In this chapter, we describe detailed protocols for the creation of a closed-chest MI model in pigs. The advantages of this model include high survival rate (>90% after ischemia–reperfusion), adjustable MI size depending on coronary occlusion site, reproducible cardiac dysfunction, and relatively low invasive method. The temporary coronary occlusion method for ischemia–reperfusion injury as well as the permanent occlusion method, using clot injection or embolic coil implantation, are described. Furthermore, we describe the key steps needed for understanding, performing, and analyzing cardiac angiography and echocardiography in pigs.

Key words

Myocardial infarction in pigs Heart failure model Ischemia–reperfusion Embolic coil Permanent coronary occlusion Thrombus Cardiac angiography in pigs Echocardiography in pigs Large animal 

Notes

Acknowledgments

This work is supported by NIH R01 HL139963 (K.I.), HL117505, HL 119046, HL129814, 128072, HL131404, HL135093, a P50 HL112324 (R.J.H.), AHA-SDG 17SDG33410873 (K.I.), and two Transatlantic Fondation Leducq grants. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health. O.B. was supported by the Deutsche Herzstiftung.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Olympia Bikou
    • 1
  • Shin Watanabe
    • 1
  • Roger J. Hajjar
    • 1
  • Kiyotake Ishikawa
    • 1
  1. 1.Cardiovascular Research CenterIcahn School of Medicine at Mount SinaiNew YorkUSA

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