Characterization of the Differential Progression of Left Ventricular Remodeling in a Rat Model of Pressure Overload Induced Heart Failure. Does Clip Size Matter?
Despite the use of inbred animals, phenotypic variability is usually encountered in rats subjected to pressure overload. This chapter describes techniques for creating a rat model of pressure overload by ascending aortic banding procedure and noninvasive characterization of the variable phenotypes by means of echocardiography. We address the variable phenotypes encountered in this model with moderate versus severe ascending aortic banding. We also describe some of the echocardiographic and hemodynamic parameters and the degree of interstitial fibrosis and extracellular matrix remodeling encountered in each of the different phenotypes.
Key wordsAscending aortic banding Pressure overload Phenotypes Hypertrophy Heart failure Myocardial remodeling Fibrosis
This work is supported by NIH R01 HL117505, HL 119046, HL129814, 128072, HL131404, R01HL135093, a P50 HL112324, and two Transatlantic Fondation Leducq grants. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health.
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