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Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture

  • Victor Gaba
  • Yehudit Tam
  • Danny Shavit
  • Benjamin Steinitz
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

Scientific photography is an important and indispensable tool in plant tissue culture research: photographs should be taken throughout a project for documentation. The aim of photography in plant tissue culture should be to illustrate clearly the differentiation, growth, and developmental stages occurring in vitro. Poor-quality scientific photography in tissue culture research and professional reports results in poor documentation. If visual aspects of the tissue culture are not well documented or not well reproduced in the image, an important part of the research is missed, the resulting report is of limited scientific value, and the research results may not be reproducible. Simple methods for improving the results of photography of materials from plant tissue culture are described and discussed, along with the necessary photographic equipment, suitable backgrounds, the construction of photographic plates, and correct use of electronic files for images. Finally, ethical concerns about image manipulation are discussed.

Key words

Photography Digital photography Plant tissue culture Plant development Image processing Image storage Image manipulation Ethical considerations 

Notes

Acknowledgments

Contribution from the Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel, No. 570/17.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Victor Gaba
    • 1
  • Yehudit Tam
    • 1
  • Danny Shavit
    • 2
  • Benjamin Steinitz
    • 3
  1. 1.Department of Plant Pathology and Weed ScienceAgricultural Research Organization—The Volcani CenterRishon LeZionIsrael
  2. 2.Shavit Professional Scientific PhotographyKfar SabaIsrael
  3. 3.Institute of Plant SciencesAgricultural Research Organization—The Volcani CenterRishon LeZionIsrael

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