Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control

  • Mona QuambuschEmail author
  • Traud Winkelmann
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)


Endophytic bacteria have been increasingly in the focus of research projects during the last decade. This has changed the view on bacteria in plant tissue culture and led to the differentiation between artificially introduced contaminations and naturally occurring endophytes with neutral, negative, or positive impact on the plant propagation process. This review chapter gives an overview on recent findings about the impact that bacteria have on the plant physiology in general and during micropropagation. Additionally, methods for the detection and identification of bacteria in plant tissue are described and, finally, suggestions of how to deal with bacterial endophytes in in vitro culture are given.

Key words

Bacteria Contamination Culture-dependent Culture-independent Endophytes Identification Micropropagation Plant growth promotion Quantification 


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Authors and Affiliations

  1. 1.Abteilung WaldgenressourcenNordwestdeutsche Forstliche VersuchsanstaltHann. MündenGermany
  2. 2.Institut für Gartenbauliche ProduktionssystemeLeibniz Universität HannoverHannoverGermany

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