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Analysis of Terpenoid Indole Alkaloids, Carotenoids, Phytosterols, and NMR-Based Metabolomics for Catharanthus roseus Cell Suspension Cultures

  • Mohd Zuwairi Saiman
  • Natali Rianika Mustafa
  • Robert Verpoorte
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

The plant Catharanthus roseus is a rich source of terpenoid indole alkaloids (TIA). Some of the TIA are important as antihypertensive (ajmalicine) and anticancer (vinblastine and vincristine) drugs. However, production of the latter is very low in the plant. Therefore, in vitro plant cell cultures have been considered as a potential supply of these chemicals or their precursors. Some monomeric alkaloids can be produced by plant cell cultures, but not on a level feasible for commercialization, despite extensive studies on this plant that deepened the understanding of the TIA biosynthesis and its regulation. In order to analyze the metabolites in C. roseus cell cultures, this chapter presents the method of TIA, carotenoids, and phytosterols analyses. Furthermore, an NMR-based metabolomics approach to study C. roseus cell culture is described.

Key words

Gas chromatography High-performance liquid chromatography (HPLC) Metabolomics Nuclear magnetic resonance (NMR) Periwinkle Plant cell suspension Tissue culture 

Notes

Acknowledgment

This work was funded by the IBOS-ACTS program as coordinated by NWO, The Netherlands. Mohd Zuwairi Saiman was sponsored by Ministry of Higher Education Malaysia and University of Malaya, Kuala Lumpur, Malaysia.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Mohd Zuwairi Saiman
    • 1
    • 2
    • 3
  • Natali Rianika Mustafa
    • 3
  • Robert Verpoorte
    • 3
  1. 1.Faculty of Science, Institute of Biological SciencesUniversity of MalayaKuala LumpurMalaysia
  2. 2.Centre for Research in Biotechnology for Agriculture (CEBAR)University of MalayaKuala LumpurMalaysia
  3. 3.Natural Products Laboratory, Institute of BiologyLeiden UniversityLeidenThe Netherlands

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