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Induction of Specialized Metabolism in In Vitro Cultures of Capsicum chinense Jacq

  • Felipe A. Vázquez-Flota
  • María de Lourdes Miranda-Ham
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

A protocol for the elicitation of capsaicinoids, the pungent principle of peppers, as well as for the biosynthetic intermediaries vanillin and ferulic acid was developed for in vitro cell suspension cultures, and immobilized placentas of Capsicum chinense Jacq. in vitro cultures were exposed to different doses of methyl jasmonate and salicylic acid, which were effective in eliciting specialized metabolism in both of these cultures, resulting in an increased accumulation of the analyzed metabolites.

Key words

Capsicum chinense Elicitation Phenolics Specialized metabolism 

References

  1. 1.
    Hartmann T (2007) From waste products to ecochemicals: Fifty years research of plant secondary metabolism. Phytochemistry 68:2831–2846. https://doi.org/10.1016/j.phytochem.2007.09.017 CrossRefGoogle Scholar
  2. 2.
    Ramírez-Estrada K, Vidal-Limón H, Hidalgo D et al (2016) Elicitation, an effective strategy of the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21:182. https://doi.org/10.3390/molecules21020182 CrossRefPubMedGoogle Scholar
  3. 3.
    Guízar-González C, Monforte-González M, Vázquez-Flota F (2016) Yeast extract induction of sanguinarine biosynthesis is partially dependent of the octadecanoic pathway in cell cultures of Argemone mexicana L., the Mexican poppy. Biotechnol Lett 38:1237–1242. https://doi.org/10.1007/s10529-016-2095-2 CrossRefGoogle Scholar
  4. 4.
    Vázquez-Flota F, Loyola-Vargas V (2003) In vitro plant cell culture as the basis for the development of a research institute in Mexico: Centro de Investigación Científica de Yucatán. In Vitro Cell Dev-Pl 39:250–258. https://doi.org/10.1079/IVP2002398 CrossRefGoogle Scholar
  5. 5.
    Vázquez-Flota F, Hernández-Domínguez E, Miranda-Ham ML, Monforte-González M (2009) A differential response to chemical elicitors in Catharanthus roseus in vitro cultures. Biotechnol Lett 31:591–595. https://doi.org/10.1007/s10529-008-9881-4 CrossRefPubMedGoogle Scholar
  6. 6.
    Baas-Espinola FM, Castro-Concha LA, Vázquez-Flota F, Miranda-Ham ML (2016) Capsaicin synthesis requires in situ phenylalanine and valine formation in in vitro maintained placentas from Capsicum chinense. Molecules 21:799. https://doi.org/10.3390/molecules21060799 CrossRefGoogle Scholar
  7. 7.
    Aldana-Iuit JG, Sauri-Duch E, Miranda-Ham ML, Castro-Concha LA, Cuevas-Glory LF, Vázquez-Flota F (2015) Nitrate promotes capsaicin accumulation in Capsicum chinense immobilized placentas. BioMed Res Intl 2015:1. https://doi.org/10.1155/2738/794084 CrossRefGoogle Scholar
  8. 8.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  9. 9.
    Monforte-González M, Medina-Lara F, Gutiérrez-Carbajal MG, Vázquez-Flota F (2007) Capsaicinoid quantitation by in situ densitometry of thin layer chromatography plates. J Liq Chromatogr Relat Technol 30:1697–1704. https://doi.org/10.1080/10826070701225041 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Felipe A. Vázquez-Flota
    • 1
  • María de Lourdes Miranda-Ham
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigación Científica de YucatánMéridaMexico

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