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Elaboration of Transcriptome During the Induction of Somatic Embryogenesis

  • Elsa Góngora-Castillo
  • Geovanny I. Nic-Can
  • Rosa M. Galaz-Ávalos
  • Víctor M. Loyola-Vargas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

Somatic embryogenesis (SE) is one of the most studied developmental processes due to its applications, such as plant micropropagation, transformation, and germplasm conservation. The use of massive techniques of sequencing, as well as the use of subtractive hybridization and macroarrays, has led to the identification of hundreds of genes involved in the SE process. These have been important developments to study the molecular aspects of the progress of SE. With the advent of the new massive techniques for sequencing RNA, it has been possible to see a more complete picture of whole processes. In this chapter we present a technique to handle the elaboration of the transcriptome from the extraction of RNA until the assembly of the complete transcriptome.

Key words

Bioinformatics Coffea canephora Somatic embryogenesis Transcriptome 

Notes

Acknowledgment

The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 1515).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Elsa Góngora-Castillo
    • 1
  • Geovanny I. Nic-Can
    • 2
  • Rosa M. Galaz-Ávalos
    • 3
  • Víctor M. Loyola-Vargas
    • 3
  1. 1.CONACYT Research Fellow-Unidad de BiotecnologíaCentro de Investigación Científica de YucatánMéridaMexico
  2. 2.CONACYT Research Fellow-Campus de Ciencias Exactas e IngenieríaUniversidad Autónoma de YucatánMéridaMexico
  3. 3.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigación Científica de YucatánMéridaMexico

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