Proteomics as a Tool to Study Molecular Changes During Plant Morphogenesis In Vitro
Proteome analysis represents a promising approach for plant tissue culture since it is now possible to identify and quantify proteins on a large scale. Biomarker discovery and the study of the molecular events associated with in vitro plant morphogenesis are considered potential targets for application of proteomics technologies. This chapter describes a protocol for application in in vitro plant material using two proteomics approaches: 2-DE coupled to mass spectrometry and liquid chromatography-linked tandem mass spectrometry.
Key wordsBioinformatics Mass spectrometry Morphogenesis Plant tissue culture Protein electrophoresis Proteomics
This work was supported by grants from São Paulo Research Foundation—FAPESP (Proc. 15/21075-4), Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro—FAPERJ (Proc. E26/201.574/2014) and National Council for Scientific and Technological Development-CNPq (Proc. 454451/2014-8).
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