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In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, cv. Curraré)

  • Rosa Maria Escobedo-Gracia-Medrano
  • Carlos Iván Cruz-Cárdenas
  • Lucila Aurelia Sánchez-Cach
  • José Roberto Ku-Cauich
  • Wilma Aracely González-Kantún
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

Most cultivated bananas (Musa spp.) are polyploids, and their fruits are seedless and propagated exclusively vegetatively; however, they can also be cloned by micropropagation techniques, viz., direct organogenesis (DO) or somatic embryogenesis (SE). Banana indirect SE (ISE), with an embryogenic callus phase, is possible using young male or female flowers as direct explant depending on the genotype or shoot tips (scalps). For the False Horn Plantain, cv. Curraré (AAB, plantain subgroup), which has a degenerating male bud, female flowers are used to regenerate plants through ISE. Here, a protocol for increasing the number of initial explant material from a single mother plant and its embryogenic response is described. For those purposes, hands with young female buds are in vitro proliferated in the presence of 1 μM indole-3-acetic acid and 2.5 μM thidiazuron. Friable embryogenic cultures, here called ISE-2, obtained from the new proliferative secondary female bud clusters are initiated on medium containing auxins. Embryogenic suspensions are then established from the ISE-2 cultures. Regeneration of plants is achieved from embryogenic suspensions after plating on semisolid medium free of plant growth regulators; greenhouse acclimatized plantlets are ready for banana farming. This study demonstrates that proliferative female buds are a proper choice for ISE.

Key words

Clonal propagation In vitro Musa spp. Plantain Plant growth regulator 

Notes

Acknowledgments

The authors would like to express their gratitude to the government of México through FORDECYT-CONACYT (Consejo Nacional de Ciencia y Tecnología) Research Project #116886 and a CONACYT-studentship No. 50323 (CICC).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rosa Maria Escobedo-Gracia-Medrano
    • 1
  • Carlos Iván Cruz-Cárdenas
    • 1
    • 2
  • Lucila Aurelia Sánchez-Cach
    • 1
  • José Roberto Ku-Cauich
    • 1
  • Wilma Aracely González-Kantún
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A.C.MéridaMexico
  2. 2.Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP)Tepatitlán de MorelosMexico

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