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Induction of Somatic Embryogenesis in Jatropha curcas

  • Rosa M. Galaz-Ávalos
  • Heydi G. Martínez-Sánchez
  • Víctor M. Loyola-Vargas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)

Abstract

Jatropha curcas has been a promising crop for biofuel production for the last decade. However, the lack of resistant materials to diseases and improved quality of the oil produced by the seeds has restricted the use of this promising crop. The genetic modifications in the fatty acid pathway, as well as the introduction of resistance to different diseases, would change the fate of Jatropha. To achieve these goals, we need to have a very efficient regeneration system. Here, we report a very useful protocol to induce somatic embryogenesis from leaves of Jatropha using cytokinin as the only growth regulator.

Key words

Auxins Cytokinins Jatropha curcas Somatic embryogenesis 

Notes

Acknowledgment

The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACYT, 1515).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rosa M. Galaz-Ávalos
    • 1
  • Heydi G. Martínez-Sánchez
    • 1
  • Víctor M. Loyola-Vargas
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de YucatánMéridaMexico

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