Induction of Somatic Embryogenesis in Jatropha curcas

  • Rosa M. Galaz-Ávalos
  • Heydi G. Martínez-Sánchez
  • Víctor M. Loyola-VargasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)


Jatropha curcas has been a promising crop for biofuel production for the last decade. However, the lack of resistant materials to diseases and improved quality of the oil produced by the seeds has restricted the use of this promising crop. The genetic modifications in the fatty acid pathway, as well as the introduction of resistance to different diseases, would change the fate of Jatropha. To achieve these goals, we need to have a very efficient regeneration system. Here, we report a very useful protocol to induce somatic embryogenesis from leaves of Jatropha using cytokinin as the only growth regulator.

Key words

Auxins Cytokinins Jatropha curcas Somatic embryogenesis 



The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACYT, 1515).


  1. 1.
    Loyola-Vargas VM, Ochoa-Alejo N (2016) Somatic embryogenesis. An overview. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis. Fundamental aspects and applications. Springer, Switzerland, pp 1–10. CrossRefGoogle Scholar
  2. 2.
    Nic-Can GI, Loyola-Vargas VM (2016) The role of the auxins during somatic embryogenesis. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis. Fundamental aspects and applications. Springer, Switzerland, pp 171–181. CrossRefGoogle Scholar
  3. 3.
    Swamy NR, Ugandhar T, Praveen M et al (2005) Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense. Ind J Biotecnol 4:414–418Google Scholar
  4. 4.
    Quiroz-Figueroa FR, Monforte-González M, Galaz-Ávalos RM et al (2006) Direct somatic embryogenesis in Coffea canephora. In: Loyola-Vargas VM, Vázquez-Flota FA (eds) Plant cell culture protocols. Humana Press, Totowa, New Jersey, pp 111–117. CrossRefGoogle Scholar
  5. 5.
    de Oliveira JS, Leite PM, de Souza LB et al (2009) Characteristics and composition of Jatropha gossypiifolia and Jatropha curcas L. oils and application for biodiesel production. Biomass Bioenergy 33:449–453. CrossRefGoogle Scholar
  6. 6.
    Barros TFS, Arriel NHC, Queiroz MF et al (2015) Fatty acid profiles of species of Jatropha curcas L., Jatropha mollissima (Pohl) Baill. And Jatropha gossypiifolia L. Ind Crop Prod 73:106–108. CrossRefGoogle Scholar
  7. 7.
    Jongschaap REE, Corré WJ, Bindraban PS et al (2007) Claims and facts on Jatropha curcas L.: global Jatropha curcas evaluation, breeding and propagation programme. Report 158. Plant Research International, Wageningen, NetherlandsGoogle Scholar
  8. 8.
    Heller J (1996) Phy. Jatropha curcas L. promoting the conservation and use of underutilized and neglected crops. Institute of Plant Genetics and Crop Plant Research/International Plant Genetic Resources Institute, Gatersleben/Rome, ItalyGoogle Scholar
  9. 9.
    Sujatha M, Reddy TP, Mahasi MJ (2008) Role of biotechnological interventions in the improvement of castor (Ricinus communis L.) and Jatropha curcas L. Biotechnol Adv 26:424–435. CrossRefPubMedGoogle Scholar
  10. 10.
    Achten WM, Nielsen LR, Aerts R et al (2010) Towards domestication of Jatropha curcas. Biofuels 1:91–107. CrossRefGoogle Scholar
  11. 11.
    Sunil N, Kumar V, Varaprasad K (2013) Origin, domestication, distribution and diversity of Jatropha curcas L. In: Bahadur B, Sujatha M, Carels N (eds) Jatropha, challenges for a new energy crop, Genetic improvement and biotechnology, vol 2. Springer, New York, pp 137–151. CrossRefGoogle Scholar
  12. 12.
    Galaz-Ávalos RM, Avilez-Montalvo RN, Ucan-Uc CM et al (2012) Jatropha curcas una alternativa para la obtención de biodiésel sin afectar el sector alimentario. Biotecnología 16:92–112Google Scholar
  13. 13.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. CrossRefGoogle Scholar
  14. 14.
    George MW, Tripepi RR (2001) Plant preservative mixture can affect shoot regeneration from leaf explants of chrysanthemum, European birch, and rhododendron. Hort Science 36:768–769Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rosa M. Galaz-Ávalos
    • 1
  • Heydi G. Martínez-Sánchez
    • 1
  • Víctor M. Loyola-Vargas
    • 1
    Email author
  1. 1.Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de YucatánMéridaMexico

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