Auxin Immunolocalization in Coffea canephora Tissues
Auxins are plant growth regulators that participate in a variety of biological mechanisms during the growth and development of plants. The most abundant natural auxin is indole-3-acetic acid (IAA). The physiological processes regulated by IAA depend on their temporal space accumulation in different tissues of a plant. This accumulation is regulated by its biosynthesis, conjugation, degradation, and transport. Therefore tools that allow us a qualitative and quantitative detection of IAA in plant tissues are very useful to understand the homeostasis of IAA during the life cycle of plants. In this protocol, the complete procedure for localization of IAA in different tissues of Coffea canephora is described using specific anti-IAA monoclonal antibodies.
Key wordsAntibody Coffea canephora Immunocytochemistry Indole-3-acetic acid Polar auxin transport
The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 257436).
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