Auxin Immunolocalization in Coffea canephora Tissues

  • Ruth E. Márquez-López
  • Ángela Ku-González
  • Hugo A. Méndez-Hernández
  • Rosa M. Galaz-Ávalos
  • Víctor M. Loyola-VargasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1815)


Auxins are plant growth regulators that participate in a variety of biological mechanisms during the growth and development of plants. The most abundant natural auxin is indole-3-acetic acid (IAA). The physiological processes regulated by IAA depend on their temporal space accumulation in different tissues of a plant. This accumulation is regulated by its biosynthesis, conjugation, degradation, and transport. Therefore tools that allow us a qualitative and quantitative detection of IAA in plant tissues are very useful to understand the homeostasis of IAA during the life cycle of plants. In this protocol, the complete procedure for localization of IAA in different tissues of Coffea canephora is described using specific anti-IAA monoclonal antibodies.

Key words

Antibody Coffea canephora Immunocytochemistry Indole-3-acetic acid Polar auxin transport 



The work from VMLV laboratory was supported by a grant received from the National Council for Science and Technology (CONACyT, 257436).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ruth E. Márquez-López
    • 1
  • Ángela Ku-González
    • 1
  • Hugo A. Méndez-Hernández
    • 1
  • Rosa M. Galaz-Ávalos
    • 1
  • Víctor M. Loyola-Vargas
    • 1
    Email author
  1. 1.Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de YucatánMéridaMexico

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