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Detection of Diagnostic Plant-Derived Psychoactive Biomarkers in Fingerprints by MALDI-SpiralTOF-Mass Spectrometry Imaging

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1810)

Abstract

One of the current challenges in forensics is establishment of a connection between an individual and substances to which they have been exposed, and which might have relevance in crime scene investigation. An example of a situation in which this arises is when an individual has handled, and is under the influence of any one of a large number of currently unscheduled plant-based mind-altering substances. In such instances as a medical emergency or a crime scene investigation , one way to establish a connection between an individual and their exposure to such substances is to take advantage of the high information content of their fingerprint. The fingerprint pattern not only establishes the identity of the individual, but also contains rarely exploited chemical information about molecules to which they have been exposed that might have a bearing on a crime. If the fingerprint image is based on the spatial distribution of diagnostic molecular markers indicative of a substance, then an individual’s identity can be definitively tied to exposure to the substance. The fingerprint image derived from the spatial distribution of diagnostic molecules can be obtained by mass spectrometry imaging (MSI). Here, we demonstrate how the handling by an individual of a plant-derived psychoactive brew called ayahuasca can be established through determination, by matrix-assisted laser desorption ionization (MALDI) MSI, of ion images featuring biomarkers from the plants from which the brew is made.

Key words

Mass spectrometry MALDI Ayahuasca Biomarkers Fingerprints Forensics 

Notes

Acknowledgments

Development of the protocol reported herein was supported in part by Award Number 2015-DN-BX-K057, awarded by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice. The opinions, findings, and conclusions or recommendations expressed in this presentation are those of the authors and do not necessarily reflect those of the Department of Justice. The support of the Department of Justice is gratefully acknowledged.

References

  1. 1.
    Statistics BoJ (2015) Bureau of Justice Statistics Drugs and Crime Facts: Drug law violations and enforcement. https://www.bjs.gov/content/dcf/enforce.cfm. Accessed 24 Feb 2017
  2. 2.
    Abuse NIoD (2016) National survey on drug use and health: trends in prevalence of various drugs for ages 12 or older, ages 12 to 17, ages 18 to 25, and ages 26 or older; 2013–2015 (in percent). https://www.drugabuse.gov/national-survey-drug-use-health. Accessed 24 Feb 2017
  3. 3.
    Administration SAaMHS (2013) Results from the 2012 national survey on drug use and health: detailed tables. Rockville, MDGoogle Scholar
  4. 4.
    Administration SAaMHS (2014) Results from the 2013 national survey on drug use and health: summary of national findings. Rockville, MDGoogle Scholar
  5. 5.
    Doering-Silveira E, Grob CS, de Rios MD, Lopez E, Alonso LK, Tacla C, Da Silveira DX (2005) Report on psychoactive drug use among adolescents using ayahuasca within a religious context. J Psychoactive Drugs 37(2):141–144. https://doi.org/10.1080/02791072.2005.10399794 CrossRefPubMedGoogle Scholar
  6. 6.
    Brierley DI, Davidson C (2012) Developments in harmine pharmacology—implications for ayahuasca use and drug-dependence treatment. Prog Neuro-Psychopharmacol Biol Psychiatry 39(2):263–272. https://doi.org/10.1016/j.pnpbp.2012.06.001 CrossRefGoogle Scholar
  7. 7.
    Loizaga-Velder A, Verres R (2014) Therapeutic effects of ritual Ayahuasca use in the treatment of substance dependence—qualitative results. J Psychoactive Drugs 46(1):63–72. https://doi.org/10.1080/02791072.2013.873157 CrossRefPubMedGoogle Scholar
  8. 8.
    McVeigh T (2014) British backpacker dies after taking hallucinogenic brew in Colombia. The Observer,Google Scholar
  9. 9.
    Sklerov J, Levine B, Moore KA, King T, Fowler D (2005) A fatal intoxication following the ingestion of 5-methoxy-N,N-dimethyltryptamine in an ayahuasca preparation. J Anal Toxicol 29(8):838CrossRefGoogle Scholar
  10. 10.
    Lesiak AD, Musah RA (2016) Application of ambient ionization high resolution mass spectrometry to determination of the botanical provenance of the constituents of psychoactive drug mixtures. Forensic Sci Int 266:271–280. https://doi.org/10.1016/j.forsciint.2016.06.009 CrossRefPubMedGoogle Scholar
  11. 11.
    Francese S, Bradshaw R, Ferguson LS, Wolstenholme R, Clench MR, Bleay S (2013) Beyond the ridge pattern: multi-informative analysis of latent fingermarks by MALDI mass spectrometry. Analyst 138(15):4215–4228. https://doi.org/10.1039/c3an36896c CrossRefPubMedGoogle Scholar
  12. 12.
    Benton M, Rowell F, Sundar L, Jan M (2010) Direct detection of nicotine and cotinine in dusted latent fingermarks of smokers by using hydrophobic silica particles and MS. Surf Interface Anal 42(5):378–385. https://doi.org/10.1002/sia.3112 CrossRefGoogle Scholar
  13. 13.
    Lewis JK, Wei J, Siuzdak G (2000) Matrix-assisted laser desorption/ionization mass spectrometry in peptide and protein analysis. In: Meyers RA (ed) Encyclopedia of analytical chemistry. Wiley, Chichester, UK, pp 5880–5894Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ChemistryState University of New York at AlbanyAlbanyUSA

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