Isolation of Rat and Mouse Alveolar Type II Epithelial Cells

  • Nicole L. Jansing
  • Jazalle McClendon
  • Hidenori Kage
  • Mitsuhiro Sunohara
  • Juan R. Alvarez
  • Zea Borok
  • Rachel L. ZemansEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1809)


The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.

Key words

Alveolar epithelium Lung digestion ATII 



This work was supported by NIH HL131608 (RLZ), the Boettcher Foundation (RLZ), and funds from the University of Colorado Denver Department of Medicine Outstanding Early Career Scholars Program (RLZ) and the Hastings Foundation (ZB).

Conflicts of Interest



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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nicole L. Jansing
    • 1
  • Jazalle McClendon
    • 1
  • Hidenori Kage
    • 2
  • Mitsuhiro Sunohara
    • 2
  • Juan R. Alvarez
    • 2
  • Zea Borok
    • 2
    • 3
    • 4
  • Rachel L. Zemans
    • 1
    • 5
    • 6
    Email author
  1. 1.Division of Pulmonary, Critical Care, and Sleep Medicine, Department of MedicineNational Jewish HealthDenverUSA
  2. 2.Division of Pulmonary, Critical Care and Sleep Medicine, Department of MedicineHastings Center for Pulmonary ResearchLos AngelesUSA
  3. 3.Department of Biochemistry and Molecular MedicineKeck School of Medicine, University of Southern CaliforniaLos AngelesUSA
  4. 4.Norris Comprehensive Cancer CenterKeck School of Medicine, University of Southern CaliforniaLos AngelesUSA
  5. 5.Division of Pulmonary Sciences and Critical Care MedicineUniversity of ColoradoDenverUSA
  6. 6.Division of Pulmonary and Critical Care Medicine, Department of MedicineUniversity of MichiganAnn ArborUSA

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