Isolation of Rat and Mouse Alveolar Type II Epithelial Cells
The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.
Key wordsAlveolar epithelium Lung digestion ATII
This work was supported by NIH HL131608 (RLZ), the Boettcher Foundation (RLZ), and funds from the University of Colorado Denver Department of Medicine Outstanding Early Career Scholars Program (RLZ) and the Hastings Foundation (ZB).
Conflicts of Interest
- 1.Dobbs LG (1990) Isolation and culture of alveolar type II cells. Am J Phys 258:L134–L147Google Scholar