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Isolation of Rat and Mouse Alveolar Type II Epithelial Cells

  • Nicole L. Jansing
  • Jazalle McClendon
  • Hidenori Kage
  • Mitsuhiro Sunohara
  • Juan R. Alvarez
  • Zea Borok
  • Rachel L. Zemans
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1809)

Abstract

The gas exchange surface of the lungs is lined by an epithelium consisting of alveolar type (AT) I and ATII cells. ATII cells function to produce surfactant, play a role in host defense and fluid and ion transport, and serve as progenitors. ATI cells are important for gas exchange and fluid and ion transport. Our understanding of the biology of these cells depends on the investigation of isolated cells. Here, we present methods for the isolation of mouse and rat ATII cells.

Key words

Alveolar epithelium Lung digestion ATII 

Notes

Acknowledgments

This work was supported by NIH HL131608 (RLZ), the Boettcher Foundation (RLZ), and funds from the University of Colorado Denver Department of Medicine Outstanding Early Career Scholars Program (RLZ) and the Hastings Foundation (ZB).

Conflicts of Interest

None.

References

  1. 1.
    Dobbs LG (1990) Isolation and culture of alveolar type II cells. Am J Phys 258:L134–L147Google Scholar
  2. 2.
    Messier EM, Mason RJ, Kosmider B (2012) Efficient and rapid isolation and purification of mouse alveolar type II epithelial cells. Exp Lung Res 38:363–373CrossRefPubMedGoogle Scholar
  3. 3.
    Gonzalez RF, Dobbs LG (2013) Isolation and culture of alveolar epithelial Type I and Type II cells from rat lungs. Methods Mol Biol 945:145–159CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Dobbs LG, Gonzalez R, Williams MC (1986) An improved method for isolating type II cells in high yield and purity. Am Rev Respir Dis 134:141–145PubMedGoogle Scholar
  5. 5.
    Dobbs LG, Mason RJ (1979) Pulmonary alveolar type II cells isolated from rats. Release of phosphatidylcholine in response to beta-adrenergic stimulation. J Clin Invest 63:378–387CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Dobbs LG, Geppert EF, Williams MC, Greenleaf RD, Mason RJ (1980) Metabolic properties and ultrastructure of alveolar type II cells isolated with elastase. Biochim Biophys Acta 618:510–523CrossRefPubMedGoogle Scholar
  7. 7.
    Demaio L, Tseng W, Balverde Z, Alvarez JR, Kim KJ, Kelley DG, Senior RM, Crandall ED, Borok Z (2009) Characterization of mouse alveolar epithelial cell monolayers. Am J Physiol Lung Cell Mol Physiol 296:L1051–L1058CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nicole L. Jansing
    • 1
  • Jazalle McClendon
    • 1
  • Hidenori Kage
    • 2
  • Mitsuhiro Sunohara
    • 2
  • Juan R. Alvarez
    • 2
  • Zea Borok
    • 2
    • 3
    • 4
  • Rachel L. Zemans
    • 1
    • 5
    • 6
  1. 1.Division of Pulmonary, Critical Care, and Sleep Medicine, Department of MedicineNational Jewish HealthDenverUSA
  2. 2.Division of Pulmonary, Critical Care and Sleep Medicine, Department of MedicineHastings Center for Pulmonary ResearchLos AngelesUSA
  3. 3.Department of Biochemistry and Molecular MedicineKeck School of Medicine, University of Southern CaliforniaLos AngelesUSA
  4. 4.Norris Comprehensive Cancer CenterKeck School of Medicine, University of Southern CaliforniaLos AngelesUSA
  5. 5.Division of Pulmonary Sciences and Critical Care MedicineUniversity of ColoradoDenverUSA
  6. 6.Division of Pulmonary and Critical Care Medicine, Department of MedicineUniversity of MichiganAnn ArborUSA

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