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Isolation and Characterization of Mouse Neutrophils

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1809)

Abstract

Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.

Key words

Neutrophils Isolation Density gradient Blood Marrow Lung 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of MedicineUniversity of Vermont College of MedicineBurlingtonUSA

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