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Intravital Microscopy in the Mouse Lung

  • Yimu Yang
  • Joseph A. Hippensteel
  • Eric P. Schmidt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1809)

Abstract

While reductionist in vitro approaches have allowed for careful interrogation of cellular pathways that underlie innate immune responses, they often fail to capture the complex multicellular interactions characteristic of acute inflammation. Intravital microscopy, by directly observing alveolar cell-cell interactions, provides unique insight into the complex intercellular mechanisms responsible for alveolar inflammation. This review discusses multiple potential approaches to intravital pulmonary imaging, with specific attention to in vivo microscopy of the freely moving mouse lung.

Key words

Microscopy Intravital Neutrophil Inflammation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yimu Yang
    • 1
  • Joseph A. Hippensteel
    • 1
  • Eric P. Schmidt
    • 1
    • 2
  1. 1.Division of Pulmonary Sciences and Critical Care Medicine, Department of MedicineUniversity of Colorado DenverAuroraUSA
  2. 2.Department of MedicineDenver Health Medical CenterDenverUSA

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