Measuring Neutrophil Bactericidal Activity
The best-known role of neutrophils is control of pathogen growth. Neutrophils contain and kill pathogens through a variety of antimicrobial activities. Regardless of the mechanism, the ability to kill pathogens is a vital outcome. This chapter describes a method to measure the in vitro bactericidal activity of isolated neutrophils as the endpoint of converging innate immune functions.
Key wordsNeutrophils Bactericidal Bacteria killing Innate immunity Reactive oxygen species Neutrophil extracellular traps Phagocytosis
This work was supported by a research grant from the Cystic Fibrosis Foundation.
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