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Measuring Neutrophil Bactericidal Activity

  • Kenneth C. Malcolm
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1809)

Abstract

The best-known role of neutrophils is control of pathogen growth. Neutrophils contain and kill pathogens through a variety of antimicrobial activities. Regardless of the mechanism, the ability to kill pathogens is a vital outcome. This chapter describes a method to measure the in vitro bactericidal activity of isolated neutrophils as the endpoint of converging innate immune functions.

Key words

Neutrophils Bactericidal Bacteria killing Innate immunity Reactive oxygen species Neutrophil extracellular traps Phagocytosis 

Notes

Acknowledgments

This work was supported by a research grant from the Cystic Fibrosis Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of MedicineNational Jewish HealthDenverUSA

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