Genipin Cross-Linking of Elastin and Elastin-Based Proteins

  • Lisa D. Muiznieks
Part of the Methods in Molecular Biology book series (MIMB, volume 1798)


Genipin is a natural plant-derived compound that covalently cross-links biopolymers into lattice networks with good biocompatibility, controllable swelling, and mechanical properties. This protocol describes the genipin cross-linking of elastic proteins, including tropoelastin and elastin-based polypeptides, through steps of elastin phase-separation upon addition of salt and heat, centrifugation to rapidly concentrate the dense protein phase, and incubation. This method is applicable for the fabrication of elastic materials suitable for use as scaffolds for biomedical applications.

Key words

Genipin Elastin Elastin-like polypeptide Cross-link Protein scaffold Hydrogel 



The author thanks Fred Keeley and Sean Reichheld for useful discussions.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Lisa D. Muiznieks
    • 1
  1. 1.Molecular Medicine ProgramResearch Institute, Hospital for Sick ChildrenTorontoCanada

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