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Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs

  • Bo Liang
  • Chi Chiu WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1797)

Abstract

In vivo pregnant study protocols covering preconceptional exposure as well as prenatal and postnatal development including the lactation period to screen the toxic effects on specific elements of the highly complicated reproductive cycle are time-consuming and expensive. Morphology-based whole embryo culture is the most common alternative in vitro embryotoxicity method to evaluate the early developmental toxicity of drugs. Here we share our procedures and experience in the whole embryo culture for drug embryotoxicity assessments.

Key words

Pharmacotoxicity Developmental toxicity In vitro embryotoxicity test Whole embryo culture 

Notes

Acknowledgments

This work was supported by General Research Fund (476210) from Research Grants Council, Hong Kong Special Administrative Regions to C.C.W, and National Natural Science Foundation of China (81303302) from National Natural Science Fund, China to B.L. and C.C.W.

References

  1. 1.
    Brown N, Spielmann H, Bechter R et al (1995) Screening chemicals for reproductive toxicity: the current alternatives. The report and recommendations of an ECVAM/ETS workshop (ECVAM workshop 12). Altern Lab Anim 23:868–882Google Scholar
  2. 2.
    Spielmann H (1998) Reproduction and development. Environ Health Perspect 106:571–576CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Maele-Fabry GV, Delhaise F, Picard JJ (1990) Morphogenesis and quantification of the development of post-implantation mouse embryo. Toxicol In Vitro 4:149–156. https://doi.org/10.1016/0887-2333(90)90037-T
  4. 4.
    Maele-Fabry GV, Delhaise F, Picard JJ (1992) Evolution of the developmental scores of sixteen morphological features in mouse embryos displaying 0 to 30 somites. Int J Dev Biol 36:161–167PubMedGoogle Scholar
  5. 5.
    Balls M (2002) Embryotoxicity/statement on the scientific validity of the postimplantation rat whole-embryo culture assay – an in vitro test for Embryotoxicity. 17th meeting of ECVAM scientific advisory committee. Altern Lab Anim 30:271–273PubMedGoogle Scholar
  6. 6.
    Genschow E, Spielmann H, Scholz G et al (2002) The ECVAM international validation study on in vitro embryotoxicity tests: results of the definitive phase and evaluation of prediction models. European Centre for the validation of alternative methods. Altern Lab Anim 30:151–176PubMedGoogle Scholar
  7. 7.
    New DA, Cockroft DL (1979) A rotating bottle culture method with continuous replacement of the gas phase. Experientia 35(1):138–140CrossRefPubMedGoogle Scholar
  8. 8.
    Ufer C, Wang CC, Fahling M et al (2008) Translational regulation of glutathione peroxidase 4 expression through guanine-rich sequence binding factor 1 is essential for embryonic brain development. Genes Dev 22:1835–1850. https://doi.org/10.1101/gad.466308 CrossRefGoogle Scholar
  9. 9.
    Wang CC, Chu KO, Chong WS et al (2007) Tea epigallocatechin-3-gallate increased 8-isoprostane level and induced caudal regression in developing rat embryos. Free Radic Biol Med 43:519–527. https://doi.org/10.1016/j.freeradbiomed.2007.04.034 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Obstetrics & Gynaecology, Li Ka Shing Institute of Health Sciences, School of Biomedical SciencesThe Chinese University of Hong KongShatinHong Kong

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