Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs
In vivo pregnant study protocols covering preconceptional exposure as well as prenatal and postnatal development including the lactation period to screen the toxic effects on specific elements of the highly complicated reproductive cycle are time-consuming and expensive. Morphology-based whole embryo culture is the most common alternative in vitro embryotoxicity method to evaluate the early developmental toxicity of drugs. Here we share our procedures and experience in the whole embryo culture for drug embryotoxicity assessments.
Key wordsPharmacotoxicity Developmental toxicity In vitro embryotoxicity test Whole embryo culture
This work was supported by General Research Fund (476210) from Research Grants Council, Hong Kong Special Administrative Regions to C.C.W, and National Natural Science Foundation of China (81303302) from National Natural Science Fund, China to B.L. and C.C.W.
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