Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs

  • Bo Liang
  • Chi Chiu WangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1797)


In vivo pregnant study protocols covering preconceptional exposure as well as prenatal and postnatal development including the lactation period to screen the toxic effects on specific elements of the highly complicated reproductive cycle are time-consuming and expensive. Morphology-based whole embryo culture is the most common alternative in vitro embryotoxicity method to evaluate the early developmental toxicity of drugs. Here we share our procedures and experience in the whole embryo culture for drug embryotoxicity assessments.

Key words

Pharmacotoxicity Developmental toxicity In vitro embryotoxicity test Whole embryo culture 



This work was supported by General Research Fund (476210) from Research Grants Council, Hong Kong Special Administrative Regions to C.C.W, and National Natural Science Foundation of China (81303302) from National Natural Science Fund, China to B.L. and C.C.W.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Obstetrics & Gynaecology, Li Ka Shing Institute of Health Sciences, School of Biomedical SciencesThe Chinese University of Hong KongShatinHong Kong

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