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Histological and Histochemical Profile for Teratological Assessment in Mus musculus

  • Maria de Lurdes PintoEmail author
  • Paula Rodrigues
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1797)

Abstract

Histological analysis of mouse embryos, fetuses, or newborns remains one the most common methods and in some cases the “gold standard” used in teratology studies to detect abnormal morphogenesis during development. Histochemistry methods are based on the selectivity of certain chemical compounds for certain cellular or tissue components, several of them related to specific maturation processes. In this chapter, specific histochemistry techniques that allow for the monitoring of critical stages of tissue and organ formation, such as skeleton and muscle differentiation, neuronal maturation, neuronal connectivity, and neurodevelopment after teratogenic exposure are described in detail.

Key words

Mouse Morphogenesis Skeleton Muscle Neurons Microscopy Histochemistry 

References

  1. 1.
    Musumeci G (2015) The old and the new concepts of histochemistry. J Histol Histopathol 2:10. https://doi.org/10.7243/2055-091X-2-10 CrossRefGoogle Scholar
  2. 2.
    Lavis LD (2011) Histochemistry: live and in color. J Histochem Cytochem 59:139–145CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    ICH (2005) Harmonised tripartite guideline S5 (R2). Detection of toxicity to reproduction for medicinal products & toxicity to male fertility. http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Safety/S5/Step4/S5_R2__Guideline.pdf. Accessed 29 Mar 2017
  4. 4.
    Makris SL et al (2009) Terminology of developmental abnormalities in common laboratory mammals (version 2). Birth Defects Res B Dev Reprod Toxicol 86:227–327CrossRefPubMedGoogle Scholar
  5. 5.
    United States Environmental Protection Agency (2017) Prenatal developmental toxicity study – health effects test guidelines OPPTS 870.3700. https://ntp.niehs.nih.gov/testing/types/dev/index.html. Accessed 29 Mar 2017
  6. 6.
    Rosenberg AE, Roth SI (2012) Bone. In: Mills SE (ed) Histology for pathologists, 4th edn. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  7. 7.
    Rigueur D, Lyons KM (2014) Whole-mount skeletal staining. In: Hilton M (ed) Skeletal development and repair, methods in molecular biology, vol 1130. Humana Press, Totowa, NJGoogle Scholar
  8. 8.
    Wilson I, Gamble M (2002) The hematoxylins and eosin. In: Theory and practice of histological techniques, 5th edn. Churchill Livingstone, LondonGoogle Scholar
  9. 9.
    Taty BA (2002) Mucins. In: Theory and practice of histological techniques, 5th edn. Churchill Livingstone, LondonGoogle Scholar
  10. 10.
    Chan K-K, Lowe J (2002) Techniques in neuropathology. In: Theory and practice of histological techniques, 5th edn. Churchill Livingstone, LondonGoogle Scholar
  11. 11.
    Bolon B, Anthony DC, Butt M et al (2008) “Current pathology techniques” symposium review: advances and issues in neuropathology. Toxicol Pathol 36(6):871–889CrossRefGoogle Scholar
  12. 12.
    Rosenberry TL, Scoggin DM (1984) Structure of human erytrocyte acetylcholinesterase. Characterization of intersubunit disulfidebonding and detergent interaction. J Biol Chem 259(9):5643–5652PubMedGoogle Scholar
  13. 13.
    Churukian CJ (2002) Pigments and minerals. In: Theory and practice of histological techniques, 5th edn. Churchill Livingstone, LondonGoogle Scholar
  14. 14.
    Naik NT (1963) Technical variations in Koelle’s histochemical method for demonstrating cholinesterase activity. Quart J Microsc Sci 104(1):89–100Google Scholar
  15. 15.
    Heffner RR Jr, Balos LL (2012) Skeletal muscle. In: Mills SE (ed) Histology for pathologists, 4th edn. Lippincott Williams & Wilkins, PhiladelphiaGoogle Scholar
  16. 16.
    Schiaffino S, Reggiani C (2011) Fiber types in mammalian skeletal muscles. Physiol Rev 91:1447–1531CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centro de Ciência Animal e Veterinária (CECAV)Universidade de Trás-os-Montes e Alto Douro (UTAD)Vila RealPortugal
  2. 2.Departamento de Ciências VeterináriasUniversidade de Trás-os-Montes e Alto Douro (UTAD)Vila RealPortugal
  3. 3.Laboratório de Histologia e Anatomia Patológica (LHAP)Universidade de Trás-os-Montes e Alto Douro (UTAD)Vila RealPortugal

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