Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii
Flagella of the unicellular green alga Chlamydomonas reinhardtii are nearly identical to cilia of vertebrate cells and provide an excellent model to study ciliogenesis. Cilia and flagella are important organelles used for motility and sensing the extracellular environment. Abnormalities in cilia structure or ciliary dysfunction can have devastating consequences ranging from diabetes and obesity to polycystic kidney disease and mental retardation. Small-molecule inhibitor libraries can be used to screen for flagellum-associated phenotypes in assembly, length, motility, deflagellation, and cellular toxicity. These phenotypes can be assessed from direct microscopic visualization and custom-designed assays. These methods identify fundamental regulators of ciliary biology as well as potential therapeutic interventions for ciliopathies.
Key wordsChemical screen Motility Chlamydomonas reinhardtii Flagellar length
We would like to thank all the members of the Avasthi Lab for the critical reading of the manuscript. This work was funded by P20 GM104936-09 (PA) and NSF GRFP 1518767 (BJ). Thanks to Soumita Dutta for providing images of healthy and contaminated cells for Fig. 1.
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