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Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma

  • Erming Tian
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1792)

Abstract

The application of fluorescence in situ hybridization (FISH) technology in diagnosis and molecular classification of cancer-risk has become an essential tool in the proceeding of personalized therapy. In multiple myeloma, the precise FISH detection of numerical and structural genetic aberrations can be carried out on metaphase chromosome spreads, interphase nuclei, and formalin fixed paraffin-embedded (FFPE) tissues. To dissect highly complex cancer genomes, a broad variety of novel DNA probes, which outpace supplies from commercial resources on the market, are also crucial to the advanced translational researches. Here, we provide the protocols for the creation of custom-made DNA probes and for conducting hybridizations on various targeting cells and tissues.

Key words

Cancer cytogenetics FISH DNA probes 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Myeloma InstituteUniversity of Arkansas for Medical SciencesLittle RockUSA

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