Cytoplasmic Immunoglobulin Vs. DNA Analysis by Flow Cytometry

  • Daisy AlapatEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1792)


Cytoplasmic immunoglobulin (CIg) vs DNA by flow cytometric (FCM) method allows us to detect DNA content of the neoplastic plasma cells with monotypic cytoplasmic immunoglobulin and also provide us with clear distinction from normal polytypic plasma cells. Abnormalities in cellular DNA content (DNA aneuploidy) and cell cycle determination (proliferative activity) can be measured rapidly by flow cytometry. FCM can measure gross differences in DNA content and distinguish a cell population with normal DNA content, or diploid from a cell population with an abnormal or aneuploid DNA content. With the dual parameter procedure a monoclonal plasma cell population can be identified down to less than 0.1–0.05% of all cells.

Key words

DNA Index Cytoplasmic immunoglobulin Kappa and lambda cIg/DNA 


  1. 1.
    Bauer KD, Clevenger CV, Endow RK et al (1986) Simultaneous nuclear antigen and DNA content quantitation using paraffin-embedded colonic tissue and multiparameter flow cytometry. Cancer Res 46:2428–2434PubMedGoogle Scholar
  2. 2.
    Barlogie B, Alexanian R, Pershouse M et al (1985) Cytoplasmic immunoglobulin content in multiple myeloma. J Clin Invest 76:765–769. CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    San Miguel JF, García-Sanz R, González M, Orfão A (1996) DNA cell content studies in multiple myeloma. Leuk Lymphoma 23:33–41. CrossRefPubMedGoogle Scholar
  4. 4.
    Chan CS, Wormsley SB, Peter JB, Schechter GP (1989) Dual parameter analysis of myeloma cells by flow cytometry. DNA content of cells containing monotypic cytoplasmic immunoglobulin. Am J Clin Pathol 91:12–17CrossRefGoogle Scholar
  5. 5.
    Barlogie B, Alexanian R, Gehan EA et al (1983) Marrow cytometry and prognosis in myeloma. J Clin Invest 72:853–861. CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Barlogie B, Alexanian R, Dixon D et al (1985) Prognostic implications of tumor cell DNA and RNA content in multiple myeloma. Blood 66:338–341PubMedGoogle Scholar
  7. 7.
    Hiddemann W, Schumann J, Andreef M et al (1984) Convention on nomenclature for DNA cytometry. Committee on Nomenclature, Society for Analytical Cytology. Cancer Genet Cytogenet 13:181–183CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PathologyUniversity of Arkansas for Medical SciencesLittle RockUSA

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