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Microfluidic Assembly of Liposomes with Tunable Size and Coloading Capabilities

  • Jessica R. Hoffman
  • Ennio Tasciotti
  • Roberto Molinaro
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1792)

Abstract

Liposomes used for the delivery of pharmaceuticals have difficulties scaling up and reaching clinical translation as they suffer from batch-to-batch variability. Here, we describe a microfluidic approach for creating reproducible, homogenous nanoparticles with tunable characteristics. These nanoparticles of sizes ranging from 30 to 500 nm are rapidly self-assembled by controlling the flow rates of ethanol and aqueous streams. This method of microfluidic assembly allows for the efficient encapsulation of both hydrophobic and hydrophilic drugs in the lipid bilayer and particle core, respectively, either separately or in combination.

Key words

Liposomes Nanomedicine Microfluidics Coloading Reproducibility Scale up 

Notes

Acknowledgments

This work was supported by grants RF-2010-2318372 and RF-2010-2305526 from the Italian Ministry of Health, William Randolph Hearst Foundation, The Regenerative Medicine Program Cullen Trust for Health Care (Project ID: 18130014), Brown Foundation (Project ID:18130011), the Hearst Foundation (Project ID: 18130017), the NIH/NCI and the Office of Research on Women’s Health (Grant # 1R56CA213859), and Cancer Prevention and Research Institute of Texas (Project ID: RP170466) to E.T. The authors acknowledge the George J. and Angelina P. Kostas Charitable Foundation and CARIPARO Foundation Ricerca Pediatrica 2016–2018 Grant.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.University of North CarolinaChapel HillUSA
  2. 2.Center of Biomimetic MedicineHouston Methodist Research InstituteHoustonUSA
  3. 3.Houston Methodist Orthopedic and Sports MedicineInstitute for Academic Medicine, Houston Methodist Research InstituteHoustonUSA
  4. 4.Department of Medicine, Cardiovascular MedicineHarvard Medical School Brigham and Women’s HospitalBostonUSA

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