Microfluidic Assembly of Liposomes with Tunable Size and Coloading Capabilities
Liposomes used for the delivery of pharmaceuticals have difficulties scaling up and reaching clinical translation as they suffer from batch-to-batch variability. Here, we describe a microfluidic approach for creating reproducible, homogenous nanoparticles with tunable characteristics. These nanoparticles of sizes ranging from 30 to 500 nm are rapidly self-assembled by controlling the flow rates of ethanol and aqueous streams. This method of microfluidic assembly allows for the efficient encapsulation of both hydrophobic and hydrophilic drugs in the lipid bilayer and particle core, respectively, either separately or in combination.
Key wordsLiposomes Nanomedicine Microfluidics Coloading Reproducibility Scale up
This work was supported by grants RF-2010-2318372 and RF-2010-2305526 from the Italian Ministry of Health, William Randolph Hearst Foundation, The Regenerative Medicine Program Cullen Trust for Health Care (Project ID: 18130014), Brown Foundation (Project ID:18130011), the Hearst Foundation (Project ID: 18130017), the NIH/NCI and the Office of Research on Women’s Health (Grant # 1R56CA213859), and Cancer Prevention and Research Institute of Texas (Project ID: RP170466) to E.T. The authors acknowledge the George J. and Angelina P. Kostas Charitable Foundation and CARIPARO Foundation Ricerca Pediatrica 2016–2018 Grant.
- 6.He C, Hu Y, Yin L, Tang C, Yin C (2010) Effects of particle size and surface charge on cellular uptake and biodistribution of polymeric nanoparticles. Biomaterials 31(13):3657–3666. https://doi.org/10.1016/j.biomaterials.2010.01.065 CrossRefPubMedGoogle Scholar