Myelin pp 67-77 | Cite as

Proteomics Analysis of Myelin Composition

  • Akihiro Ishii
  • David Han
  • Rashmi BansalEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)


The identification and functional characterization of the repertoire of myelin proteins provides a valuable foundation for understanding molecular mechanisms of myelination and the pathogenesis of human myelin disease. Here we provide a procedure for the purification of myelin from rodent or human brains and a large-scale analysis of the myelin proteome, using the shotgun approach of one-dimensional PAGE and liquid chromatography (LC)/tandem mass spectrometry (MS).

Key words

Myelin Proteomics Tandem mass spectrometry Liquid chromatography Sucrose gradient 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeuroscienceUniversity of Connecticut Medical SchoolFarmingtonUSA

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