Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry
Lipids are essential components of cells and tissues. They play active and central roles in signaling and many biological functions and therefore their dysregulation is very often the first signal of function alteration. Here we describe the protocol to analyze not only lipid expression in rat sciatic nerve but also the lipid distribution along its different anatomic areas. The protocol combines results from MALDI-IMS and UHPLC-MS/MS to identify and cartography the maximum number of lipid species in the tissue.
Key wordsLipidomics Imaging mass spectrometry UHPLC MALDI Sciatic nerve
Work in the lab of the authors was funded by grants from the Basque Government (IT-971-16 and KK2016-00036 to BO) and the Spanish Ministry of Innovation, Economy and Competitiveness (SAF2015-64352 to BO and RTC-2015-3693-1 to JAF and BO). Technical and human support provided by the Servicio de Lipidómica of the SGIKER (UPV/EHU, MICINN, GV/EJ, ESF) is gratefully acknowledged. JG thanks the UPV/EHU for a pre-doctoral fellowship.
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