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Myelin pp 287-292 | Cite as

Efficient Gene Transfer to Myelinating Schwann Cells in the Rodent Sciatic Nerve Using In Vivo Electroporation

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

To study the signaling mechanism of the development and maintenance of myelinating Schwann cells (SCs) in the peripheral nervous system, in vivo techniques for SC-selective gene manipulation are useful. The present protocol describes an in vivo electroporation method that allows efficient transfection of myelinating SCs in rodent sciatic nerves. This technique allows us to introduce the genes of interest into myelinating SCs by simply applying electric pulses to the sciatic nerve after plasmid DNA injection.

Key words

Schwann cell Sciatic nerve Myelin sheath In vivo gene transfer Electroporation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKENOsakaJapan
  2. 2.Department of Pharmacology, Graduate School of MedicineThe University of TokyoTokyoJapan
  3. 3.Division of Cellular and Molecular PharmacologyNihon University School of MedicineTokyoJapan

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