Myelin pp 251-262 | Cite as

Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve

  • Xin-Peng Dun
  • David B. ParkinsonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)


Injury to the peripheral nervous system begins a well-characterized process within both neurons and Schwann cells to allow axonal regrowth, remyelination, and functional repair. Models of peripheral nerve injury have been widely used to study the behavior of Schwann cells, neurons, and other cell types such as macrophages as the events of Wallerian degeneration and regeneration take place. The most commonly used approaches in rodent models to model nerve injury in human patients are sciatic nerve transection and nerve crush, and both have well established time courses of demyelination, immune cell influx, axonal regrowth, and remyelination. We describe the techniques of sciatic nerve surgery for transection and crush injury, together with methods for the analysis of events within peripheral nerve repair in these two models.

Key words

Peripheral nerve Sciatic Nerve transection Nerve crush Surgery Demyelination Remyelination Schwann cell 


  1. 1.
    Zochodne DW (2012) The challenges and beauty of peripheral nerve regrowth. J Peripher Nerv Syst 17(1):1–18. Scholar
  2. 2.
    Parrinello S, Napoli I, Ribeiro S, Wingfield Digby P, Fedorova M, Parkinson DB, Doddrell RD, Nakayama M, Adams RH, Lloyd AC (2010) EphB signaling directs peripheral nerve regeneration through Sox2-dependent Schwann cell sorting. Cell 143(1):145–155. Scholar
  3. 3.
    Dun XP, Parkinson DB (2015) Visualizing peripheral nerve regeneration by whole mount staining. PLoS One 10(3):e0119168. Scholar
  4. 4.
    Cattin AL, Burden JJ, Van Emmenis L, Mackenzie FE, Hoving JJ, Garcia Calavia N, Guo Y, McLaughlin M, Rosenberg LH, Quereda V, Jamecna D, Napoli I, Parrinello S, Enver T, Ruhrberg C, Lloyd AC (2015) Macrophage-induced blood vessels guide Schwann cell-mediated regeneration of peripheral nerves. Cell 162(5):1127–1139. Scholar
  5. 5.
    Cattin AL, Lloyd AC (2016) The multicellular complexity of peripheral nerve regeneration. Curr Opin Neurobiol 39:38–46. Scholar
  6. 6.
    Yang DP, Zhang DP, Mak KS, Bonder DE, Pomeroy SL, Kim HA (2008) Schwann cell proliferation during Wallerian degeneration is not necessary for regeneration and remyelination of the peripheral nerves: axon-dependent removal of newly generated Schwann cells by apoptosis. Mol Cell Neurosci 38(1):80–88. S1044-7431(08)00050-X [pii]CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Arthur-Farraj PJ, Latouche M, Wilton DK, Quintes S, Chabrol E, Banerjee A, Woodhoo A, Jenkins B, Rahman M, Turmaine M, Wicher GK, Mitter R, Greensmith L, Behrens A, Raivich G, Mirsky R, Jessen KR (2012) C-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration. Neuron 75(4):633–647. S0896-6273(12)00583-1 [pii]CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Mindos T, Dun XP, North K, Doddrell RD, Schulz A, Edwards P, Russell J, Gray B, Roberts SL, Shivane A, Mortimer G, Pirie M, Zhang N, Pan D, Morrison H, Parkinson DB (2017) Merlin controls the repair capacity of Schwann cells after injury by regulating Hippo/YAP activity. J Cell Biol 216(2):495–510. Scholar
  9. 9.
    Roberts SL, Dun XP, Doddrell RDS, Mindos T, Drake LK, Onaitis MW, Florio F, Quattrini A, Lloyd AC, D’Antonio M, Parkinson DB (2017) Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve. Development 144(17):3114–3125.
  10. 10.
    Roberts SL, Dun XP, Dee G, Gray B, Mindos T, Parkinson DB (2016) The role of p38alpha in Schwann cells in regulating peripheral nerve myelination and repair. J Neurochem 141:37. Scholar
  11. 11.
    Siconolfi LB, Seeds NW (2001) Mice lacking tPA, uPA, or plasminogen genes showed delayed functional recovery after sciatic nerve crush. J Neurosci 21(12):4348–4355CrossRefPubMedGoogle Scholar
  12. 12.
    Hargreaves K, Dubner R, Brown F, Flores C, Joris J (1988) A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 32(1):77–88CrossRefPubMedGoogle Scholar
  13. 13.
    Inserra MM, Bloch DA, Terris DJ (1998) Functional indices for sciatic, peroneal, and posterior tibial nerve lesions in the mouse. Microsurgery 18(2):119–124.<119::AID-MICR10>3.0.CO;2-0. [pii]CrossRefPubMedGoogle Scholar
  14. 14.
    Baptista AF, Gomes JR, Oliveira JT, Santos SM, Vannier-Santos MA, Martinez AM (2007) A new approach to assess function after sciatic nerve lesion in the mouse—adaptation of the sciatic static index. J Neurosci Methods 161(2):259–264. Scholar
  15. 15.
    Napoli I, Noon LA, Ribeiro S, Kerai AP, Parrinello S, Rosenberg LH, Collins MJ, Harrisingh MC, White IJ, Woodhoo A, Lloyd AC (2012) A central role for the ERK-signaling pathway in controlling Schwann cell plasticity and peripheral nerve regeneration in vivo. Neuron 73(4):729–742. S0896-6273(12)00035-9 [piiCrossRefPubMedGoogle Scholar
  16. 16.
    Mogha A, Harty BL, Carlin D, Joseph J, Sanchez NE, Suter U, Piao X, Cavalli V, Monk KR (2016) Gpr126/Adgrg6 has Schwann cell autonomous and nonautonomous functions in peripheral nerve injury and repair. J Neurosci 36(49):12351–12367. Scholar
  17. 17.
    Quintes S, Brinkmann BG, Ebert M, Frob F, Kungl T, Arlt FA, Tarabykin V, Huylebroeck D, Meijer D, Suter U, Wegner M, Sereda MW, Nave KA (2016) Zeb2 is essential for Schwann cell differentiation, myelination and nerve repair. Nat Neurosci 19(8):1050–1059. Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Plymouth University Peninsula Schools of Medicine and DentistryPlymouthUK

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