Myelin Protein Zero180–199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice
Mouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0)180–199 peptide in combination with Freund’s complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.
Key wordsExperimental autoimmune neuritis Myelin P0 Peripheral neuropathy C57BL/6 mice
This work was supported by an Australian National Health and Medical Research Project Grant #APP1058647 to Xiao J.
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