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Myelin pp 243-250 | Cite as

Myelin Protein Zero180–199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice

  • David G. Gonsalvez
  • SangWon Yoo
  • Georgina A. Craig
  • Rhiannon J. Wood
  • Jessica L. Fletcher
  • Simon S. Murray
  • Junhua Xiao
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

Mouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0)180–199 peptide in combination with Freund’s complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.

Key words

Experimental autoimmune neuritis Myelin P0 Peripheral neuropathy C57BL/6 mice 

Notes

Acknowledgments

This work was supported by an Australian National Health and Medical Research Project Grant #APP1058647 to Xiao J.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • David G. Gonsalvez
    • 1
  • SangWon Yoo
    • 1
  • Georgina A. Craig
    • 1
  • Rhiannon J. Wood
    • 1
  • Jessica L. Fletcher
    • 1
  • Simon S. Murray
    • 1
  • Junhua Xiao
    • 1
  1. 1.Faculty of Medicine, Dentistry and Health Sciences, Department of Anatomy and Neuroscience, School of Biomedical SciencesThe University of MelbourneMelbourneAustralia

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