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Myelin pp 233-241 | Cite as

Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

  • Chenyi Wang
  • Mark R. Kotter
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

Multiple sclerosis (MS) is a chronic autoimmune, inflammatory disease in the central nervous system (CNS) characterized by loss of oligodendrocytes, myelin axons, and neurons. Remyelination is an endogenous repair mechanism, which recovers the loss of myelin and is able to preserve functional axons. The hope is that the development of new treatments aiming at promoting remyelination will halt and potentially partially reverse the progressive neurological decline in MS. The development of such drugs requires adequate models. In this chapter, we will discuss the surgical procedure of injection of lysolecithin into ventral thoraco-lumbar spinal cord white matter of mice, which is particularly suitable for investigating remyelination using transgenic animals.

Key words

Demyelination Remyelination Mouse model Lysolecithin Oligodendrocyte precursor cell 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Neurosciences, Anne McLaren LaboratoryUniversity of CambridgeCambridgeUK

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