Myelin pp 227-232 | Cite as

Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35–55 in the Mouse

  • Mercedes Giralt
  • Amalia Molinero
  • Juan HidalgoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)


Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35–55) + complete Freund’s adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG35–55.

Key words

Experimental autoimmune encephalomyelitis EAE Myelin oligodendrocyte glycoprotein MOG35–55 Multiple sclerosis 



The authors are grateful to grants SAF2011-23272 and SAF2014-56546-R (Ministerio de Economía y Competitividad y Fondo Europeo de Desarrollo Regional) to J.H.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Mercedes Giralt
    • 1
    • 2
  • Amalia Molinero
    • 1
    • 2
  • Juan Hidalgo
    • 1
    • 2
    Email author
  1. 1.Institute of Neurosciences, Universitat Autònoma de BarcelonaBarcelonaSpain
  2. 2.Department of Cellular Biology, Physiology and ImmunologyUniversitat Autònoma de Barcelona, BellaterraBarcelonaSpain

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