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Myelin pp 207-224 | Cite as

Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro

  • Agata Blasiak
  • Sudip Nag
  • In Hong Yang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive—new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.

Key words

Activity-dependent myelination Oligodendrocytes Neurons Optogenetics Subcellular stimulation Microfluidics Compartmentalized culture In vitro myelination 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Singapore Institute for NeurotechnologyNational University of SingaporeSingaporeSingapore
  2. 2.Department of Electronics and Electrical Communication EngineeringIndian Institute of TechnologyKharagpurIndia
  3. 3.Department of Biomedical Engineering, School of MedicineJohns Hopkins UniversityBaltimoreUSA

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