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Myelin pp 169-177 | Cite as

Modeling CNS Myelination Using Micropillar Arrays

  • Nan-Xing Huang
  • Yun-An A. Shen
  • Feng Mei
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1791)

Abstract

Myelination necessitates axons to initiate concentric membrane wrapping by oligodendroglia in the CNS. Here, we describe an in vitro system that models CNS myelination with a minimally permissive environment, termed Binary Indicant for myelination using Micropillar Arrays (BIMA). Engineered with conical micropillar arrays, BIMA allows for rapid translation of oligodendroglial wrapping and differentiation into binary readout under confocal microscopy. Fabricated into 96- or 384-well plates, BIMA serves as a high-throughput screening platform for compounds that may promote oligodendroglial differentiation and myelination. BIMA is also amenable for examining molecular signals and pathways that regulate axon-glia interaction and recognition.

Key words

Myelin basic protein Oligodendroglia Oligodendrocyte precursor cell Oligodendrocyte Platelet-derived growth factor receptor alpha Myelin 

Notes

Acknowledgments

We thank Dr. Jonah R. Chan (UCSF) for the advice and editing on the chapter. This work was supported partially by the National Natural Science Foundation of China (NSCF31771120, 31471043).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Histology and Embryology, Chongqing Key Laboratory of NeurobiologyThird Military Medical UniversityChongqingChina
  2. 2.Department of Neurology and Program in NeuroscienceUniversity of CaliforniaSan FranciscoUSA
  3. 3.Department of NeuroscienceGenentech Inc.South San FranciscoUSA

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