Electron Microscopy for the Analysis of Peripheral Nerve Myelin
Transmission electron microscopy (TEM) can provide excellent high-resolution images of cellular structures. A critical step with this technique is sample preparation. Here we describe our protocol for the preparation of semithin and ultrathin sections of mouse peripheral nerves in order to visualize the structure and pathological features of peripheral myelin with TEM.
Key wordsMyelin Schwann cell Periodicity TEM Semithin sectioning Ultrathin sectioning Sample preparation
This work was supported by Fondazione Telethon (GGP14147) and the Italian Ministry of Health (GR-2011-0234679).
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