Optical Bioluminescence Protocol for Imaging Mice
The presence, growth, or decline of transfected cell populations expressing the enzyme Luciferase can be followed in live mice using bioluminescence optical imaging techniques. This protocol describes how to verify the imaging equipment, options for injecting the substrate Luciferin into mice, image acquisition considerations, and commonly used data analysis techniques.
Key wordsBioluminescence Luciferin Luciferase Optical imaging Mouse Oncology Tumor imaging
This work was made possible by numerous faculty, student, and staff members at the UCLA Crump Institute for Molecular Imaging Preclinical Technology Imaging Center. Waldemar Ladno, DVM and Darin Williams were invaluable with veterinary and logistical help with operations, education, and research work.
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