Macrophages pp 259-262 | Cite as

Depleting Macrophages In Vivo with Clodronate-Liposomes

  • Stéphanie G. MorenoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1784)


In order to decipher the role of macrophages in vivo, it could be necessary to establish a model of macrophage depletion in the whole animal. One method to obtain animal models efficiently depleted in macrophages in different tissues (bone marrow, spleen, liver, lungs, brain, gut, peritoneal cavity, lymph nodes/vessels) and blood is the use of a clodronate-liposome solution.

Here, we describe the protocol used to deplete efficiently macrophages in mouse bone marrow.

Key words

Macrophages Clodronate Bone marrow Retro-orbital injection 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.CEA/DRF/IBFJ/iRCM/LRTSFontenay aux RosesFrance
  2. 2.INSERM U967Fontenay aux RosesFrance
  3. 3.Université Paris-DiderotParisFrance
  4. 4.Université Paris-SudOrsayFrance
  5. 5.Equipe labellisée Ligue contre le CancerFontenay aux RosesFrance

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