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Macrophages pp 111-117 | Cite as

Activating Murine Macrophages In Vitro

  • Elizabeth Dalby
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1784)

Abstract

Macrophage activation is a process that is highly relevant in both infectious and chronic diseases and so the study of macrophage activation is of high interest to investigators in many fields of biomedical research. Bone marrow-derived macrophages are a popular choice for studying macrophage activation in vitro, as these cells are relatively hardy and many macrophages can be produced from just one mouse. Here we describe the activation of mouse macrophages in vitro, including special considerations for cell culture, detachment, kinetics, and activation states.

Key words

Macrophages M1 Macrophage activation BMDMs Lipopolysaccharide Interferon gamma Classically activated macrophages 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Elizabeth Dalby
    • 1
  1. 1.BioStat Solutions Inc.FrederickUSA

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