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Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics

  • Desalegn W. Etalo
  • Carmen Díez-Simón
  • Ric C. H. de Vos
  • Robert D. HallEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1778)

Abstract

There is increasing demand to bring the level of metabolomics analyses down to the tissue or cell level. Significant progress has been made involving the use of in situ metabolomics imaging techniques where no tissue collection or extraction is needed prior to analysis. In this chapter we describe a relatively new method which is simple and easy to use. No ectopic matrix or vacuum is required, and analyses are performed with living plant materials directly from (or even still attached to) the plant. Although relatively straightforward, there are still a few caveats as regards this method which are described at the end of the chapter.

Key words

LAESI Laser ablation ESI-MS In situ metabolomics Spatial resolution Plant metabolomics Imaging 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Desalegn W. Etalo
    • 1
  • Carmen Díez-Simón
    • 2
  • Ric C. H. de Vos
    • 3
    • 4
  • Robert D. Hall
    • 2
    • 3
    • 4
    Email author
  1. 1.Netherlands Institute of EcologyWageningenThe Netherlands
  2. 2.Laboratory of Plant PhysiologyWageningen University and ResearchWageningenThe Netherlands
  3. 3.Business Unit BioscienceWageningen University and ResearchWageningenThe Netherlands
  4. 4.Netherlands Metabolomics CentreLeidenThe Netherlands

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