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An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones

  • Ondřej NovákEmail author
  • Kristýna Floková
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1778)

Abstract

The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS).

Key words

Phytohormones Jasmonates Auxins Abscisic acid Salicylic acid Solid-phase extraction (SPE) Liquid chromatography–mass spectrometry LC-MS 

Notes

Acknowledgments

We thank Karel Doležal and Aleš Pěnčík for critical reading of the protocol and Helena Mazáčová for careful revision of the manuscript. This work was funded by the Czech Science Foundation (Nr. GA17-06613S).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural ResearchInstitute of Experimental Botany of the Czech Academy of SciencesOlomoucCzech Republic
  2. 2.Faculty of SciencePalacký UniversityOlomoucCzech Republic
  3. 3.Plant Hormone Biology, Swammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamThe Netherlands

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