An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones
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The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS).
Key wordsPhytohormones Jasmonates Auxins Abscisic acid Salicylic acid Solid-phase extraction (SPE) Liquid chromatography–mass spectrometry LC-MS
We thank Karel Doležal and Aleš Pěnčík for critical reading of the protocol and Helena Mazáčová for careful revision of the manuscript. This work was funded by the Czech Science Foundation (Nr. GA17-06613S).
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