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Preparation and Characterization of Tau Oligomer Strains

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Amyloid Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1779))

Abstract

An increasing number of studies have demonstrated the existence of multiple conformational entities of tau, as have been observed for prion protein. We have developed and optimized techniques to isolate and study oligomeric tau strains both in vitro and ex vivo. Moreover, we have modified protocols that demonstrate the seeding properties of oligomeric tau strains that are capable of propagating in vivo. These methods and protocols are explained in this chapter.

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Acknowledgment

We thank Kayed lab members for their contributions in developing and optimizing the protocols and providing us with their helpful suggestions. This work was supported by grants from NIH RF1AG055771, RO1AG054025 and RO1NS094557, and The Gillson Longenbaugh Foundation.

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Authors and Affiliations

  1. George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, USA

    Urmi Sengupta, Mariana Carretero-Murillo & Rakez Kayed

  2. Department of Neurology, University of Texas Medical Branch, Galveston, TX, USA

    Urmi Sengupta, Mariana Carretero-Murillo & Rakez Kayed

  3. Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA

    Urmi Sengupta, Mariana Carretero-Murillo & Rakez Kayed

Authors
  1. Urmi Sengupta
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  2. Mariana Carretero-Murillo
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  3. Rakez Kayed
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Corresponding author

Correspondence to Rakez Kayed .

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Editors and Affiliations

  1. Department of Neuroscience and Physiology, New York University School of Medicine, New York University Langone Health, New York, New York, USA

    Einar M. Sigurdsson

  2. Chronic Disease Programme-CROSADIS CIBERNED, Queen Sofia Foundation, Alzheimer Center CIEN Foundation, Instituto de Salud Carlos III, Madrid, Spain

    Miguel Calero

  3. Institute of Physical Chemistry “Rocasolano”, Spanish National Research Council (CSIC), Madrid, Spain

    María Gasset

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Sengupta, U., Carretero-Murillo, M., Kayed, R. (2018). Preparation and Characterization of Tau Oligomer Strains. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 1779. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7816-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7816-8_9

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  • Print ISBN: 978-1-4939-7815-1

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