Abstract
Luminescent-conjugated oligo- and polythiophenes (LCOs and LCPs) are valuable tools for optical imaging of a plethora of protein aggregates associated with amyloidoses. Here, we outline updated protocols for the application of the anionic pentameric LCO, p-FTAA, for staining and hyperspectral imaging of protein aggregates in a variety of settings such as in vitro formed amyloid fibrils, ex vivo tissue sections, and whole brain Drosophila.
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Acknowledgment
This work was supported by the Swedish Research Council (P.H.), Linköping University (P.H., K.P.R.N., M.L.), The Swedish Foundation for Strategic Research (K.P.R.N.), and Göran Gustafsson Foundation (P.H.). We thank Maria Jonson for the spectral image of Drosophila brain and Daniel Sjölander for the images of TTR cardiac amyloid.
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Nilsson, K.P.R., Lindgren, M., Hammarström, P. (2018). Luminescent-Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues. In: Sigurdsson, E., Calero, M., Gasset, M. (eds) Amyloid Proteins. Methods in Molecular Biology, vol 1779. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7816-8_30
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DOI: https://doi.org/10.1007/978-1-4939-7816-8_30
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