Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery
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Cell penetrating peptides (CPPs) have been proven to be an effective vector to deliver a variety of membrane-impermeable macromolecules, such as DNAs, siRNAs, and proteins. Conventional single-chain CPPs typically suffer from severe protease degradation and fast clearance rate for in vivo therapeutic delivery application. In this chapter, we show that supramolecular assembly of de novo designed cationic multidomain peptides (MDPs) leads to nanostructured filaments with increased proteolytic stability and potent membrane activity necessary for improved transfection efficiency.
Key wordsSelf-assembly Cell penetrating peptide Gene delivery Membrane activity
Clarkson University is acknowledged for the support of this work. We thank the Clarkson-Trudeau Partnership for providing seed fund to support this project. This study was supported by the National Science Foundation (DMR 1654426).