Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials

  • Hyo-Eon JinEmail author
  • Seung-Wuk Lee
Part of the Methods in Molecular Biology book series (MIMB, volume 1776)


M13 bacteriophages have several qualities that make them attractive candidates as building blocks for tissue regenerating scaffold materials. Through genetic engineering, a high density of functional peptides and proteins can be simultaneously displayed on the M13 bacteriophage’s outer coat proteins. The resulting phage can self-assemble into nanofibrous network structures and can guide the tissue morphogenesis through proliferation, differentiation and apoptosis. In this manuscript, we will describe methods to develop major coat-engineered M13 phages as a basic building block and aligned tissue-like matrices to develop regenerative nanomaterials.

Key words

M13 bacteriophage Nanofiber Phage engineering Tissue regenerating materials Self-assembly 



This work was supported under the framework of international cooperation program managed by the National Research Foundation of Korea (NRF-2016K2A9A1A01951919). H.-E.J. was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2016R1C1B1008824). We acknowledge funding support from the Tsinghua-Berkeley Shenzhen Institute.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BioengineeringUniversity of California, BerkeleyBerkeleyUSA
  2. 2.Biological Systems and EngineeringLawrence Berkeley National LaboratoryBerkeleyUSA
  3. 3.Tshinghua Berkeley Shenzhen InstituteBerkeleyUSA
  4. 4.College of PharmacyAjou UniversitySuwonKorea

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