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Presenting Peptides at the Surface of Potyviruses In Planta

  • Flora Sánchez
  • Fernando Ponz
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1776)

Abstract

Potyviruses are plant viruses with elongated, flexuous virions amenable to modifications in the only viral structural protein, the coat protein (CP). Out of the several theoretically possible modifications to the CP, the one most exploited for peptide presentation is the genetic fusion of the peptide-to-be-expressed, to the CP N-terminus. Successful high-level expression of the modified CP has been achieved this way. The purified recombinant viral particles incorporate most, if not all, the properties of the expressed peptides. For many purposes, the recombinant virus particles present in extracts of infected plants should be purified for further use. Procedures for carrying out the whole process, from cloning to purification are described in the chapter.

Key words

Potyvirus Recombinant CP Peptide presentation Virion purification 

Notes

Acknowledgments

The authors thank Ivonne González-Gamboa, Carmen Yuste-Calvo, Papaiah Sardaru, and Silvia López-González for their generous gift of graphic material. The technical support of Lucía Zurita is deeply acknowledged. Work about nanobiotechnological developments of TuMV has been funded over the years by several INIA grants.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centro de Biotecnología y Genómica de Plantas (CBGP, UPM-INIA)MadridSpain

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