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Drug-Loaded Plant-Virus Based Nanoparticles for Cancer Drug Delivery

  • Michael A. Bruckman
  • Anna E. Czapar
  • Nicole F. SteinmetzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1776)

Abstract

Nature has designed nanosized particles, specifically viruses, equipped to deliver cargo to cells. We report the chemical bioconjugation and shape shifting of a hollow, rod-shaped tobacco mosaic virus (TMV) to dense spherical nanoparticles (SNPs). We describe methods to transform TMV rods to spheres, load TMV rods and spheres with the chemotherapeutic drug, doxorubicin (DOX), to deliver modified particles to breast cancer cells, and to determine the IC50 values of the plant virus-based drug delivery system.

Key words

Tobacco mosaic virus (TMV) Viral nanoparticles (VNPs) Bioconjugation Doxorubicin (DOX) Drug delivery Breast cancer 

Notes

Acknowledgments

This work was supported in part by a grant from the National Science Foundation (DMR 1452257 to N.F.S.).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Michael A. Bruckman
    • 1
    • 2
  • Anna E. Czapar
    • 3
  • Nicole F. Steinmetz
    • 1
    • 4
    • 5
    • 6
    • 7
    Email author
  1. 1.Department of Biomedical EngineeringCase Western Reserve UniversityClevelandUSA
  2. 2.NanoBio SystemsElyriaUSA
  3. 3.Department of PathologyCase Western Reserve UniversityClevelandUSA
  4. 4.Department of RadiologyCase Western Reserve UniversityClevelandUSA
  5. 5.Department of Materials Science and EngineeringCase Western Reserve UniversityClevelandUSA
  6. 6.Department of Macromolecular Science and EngineeringCase Western Reserve UniversityClevelandUSA
  7. 7.Case Comprehensive Cancer CenterCase Western Reserve UniversityClevelandUSA

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