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Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians

  • Markus A. Grohme
  • Miquel Vila-Farré
  • Jochen C. RinkEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1774)

Abstract

High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.

Key words

Planarian Genomic DNA Chaotropic salt CTAB Organic phase extraction Next-generation sequencing 

Notes

Acknowledgment

We would like to thank Sylke Winkler of the DNA sequencing core facility at the MPI-CBG for assistance with DNA quality control and quantification.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Markus A. Grohme
    • 1
  • Miquel Vila-Farré
    • 1
  • Jochen C. Rink
    • 1
    Email author
  1. 1.Max Planck Institute of Molecular Cell Biology and GeneticsDresdenGermany

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