Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians
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High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.
Key wordsPlanarian Genomic DNA Chaotropic salt CTAB Organic phase extraction Next-generation sequencing
We would like to thank Sylke Winkler of the DNA sequencing core facility at the MPI-CBG for assistance with DNA quality control and quantification.
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