Tissue Transplantations in Planarians
Tissue transplantation is an important tool for in vivo studies of pattern organization and axis establishment or maintenance in planarians. Further, transplants can inform on cell movements under different regenerative conditions or genetic backgrounds. Here, we detail classical grafting strategies that have been variously employed in planarian research over the last few decades. These protocols remain similarly useful for addressing current and future questions in the field and the step-by-step protocol that we provide allows facile adaptation of this important method into the experimental repertoire of a laboratory.
Key wordsPlanarian Transplantation Graft Tissue Axis Patterning Neoblast
This work was supported by grant BFU2011-22786 (Ministerio de Economía y Competitividad, Spain) to E.S., and grant 2014 SGR 687 (AGAUR) to E.S. J.I.R-L received a FPI fellowship from the Ministerio de Educación y Ciencia (Spain).
Video showing the different steps to proceed in the host immobilization (Sarcophagus) (MP4 135851 kb)
- 2.Schilt J (1970) Induction expèrimentale d’excroissances par des greffes hétéropolaires chez la planaire Dugesia lugubris O. Schimdt. Ann Embryol Morphogenet 3:93–106Google Scholar
- 8.Baguñà J, Saló E, Auladell C (1989) Regeneration and pattern formation in planarians III. Evidence that neoblasts are totipotent stem cells and the source of blastema cells. Development 107:77–86Google Scholar