Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections
Planarians are flatworms with almost unlimited regenerative abilities, which make them an excellent model for stem cell-based regeneration. To study the process of regeneration at the cellular level, immunohistochemical staining methods are an important tool, and the availability of such protocols is one of the prerequisites for mechanistic experiments in any animal model. Here, we detail protocols for paraffin embedding and immunostaining of paraffin sections of the model species Schmidtea mediterranea. This protocol yields robust results with a variety of commercially available antibodies. Further, the procedures provide a useful starting point for customizing staining procedures for new antibodies and/or different planarian species.
Key wordsPlanarian Immunohistochemistry Paraffin histological sections Antibody Schmidtea mediterranea
This work was supported by grant BFU2011-22786 (Ministerio de Economía y Competitividad, Spain) to TA, grant BFU2012-31701 (Ministerio de Economía y Competitividad, Spain) to FC. MA-C received an FPI fellowship from the Ministerio de Educación y Ciencia (Spain); SB and MS-G received an FI fellowship from the Generalitat de Catalunya; and NS received an APIF fellowship from the University of Barcelona.