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Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells

  • Anne-Laure Hafner
  • Tala Mohsen-Kanson
  • Christian DaniEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1773)

Abstract

In this chapter, we describe a protocol to induce at a high rate the differentiation of brown/brown like adipocyte progenitors (BAPs) derived from human induced pluripotent stem cells (hiPSCs). We also describe culture conditions to maintain hiPSCs and to derive hiPSC-BAPs.

This novel culture system provides an unlimited source of human brown adipocytes and a unique means for studying events regulating the generation and recruitment of human BAPs.

Key words

Human induced pluripotent stem cells Brown adipocytes Adipocyte Differentiation Adipocyte progenitors 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Anne-Laure Hafner
    • 1
  • Tala Mohsen-Kanson
    • 1
    • 2
  • Christian Dani
    • 1
    Email author
  1. 1.Faculté de MédecineUniversité Nice Sophia Antipolis, iBV, UMR CNRS/INSERMNice Cedex 2France
  2. 2.Department of Biology, Faculty of Sciences IILebanese UniversityFanarLebanon

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